Figure 1.

Different stem-loop primers were designed to distinguish let-7 family members. (A) Multiple sequence alignment of nine let-7 family members was performed to analyse the conserved nature of let-7 family. (B-J) Sequences of the specific stem-loop primers for let-7 family members, including (B) let-7a, (C) let-7b, (D) let-7d, (E) let-7d, (F) let-7e, (G) let-7f, (H) let-7g, (I) let-7i and (J) miR-98. Red underscored characters indicated variant nucleotides that differ from let-7a. F, forward; Hsa, Homo sapiens; R, Reverse; uni, universal.