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Specificity of the modified stem-loop RT-PCR assay. Mature let-7a specific qPCR was conducted by adding synthesized analogues of let-7b and let-7c as interference. The indicated amounts of synthetic let-7a, let-7b and let-7c were mixed together and reverse transcribed with mixed RT primers prior to RT-qPCR **P<0.01. RT-qPCR, reverse transcription-quantitative polymerase chain reaction.
