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. 2018 Jan 5;17(3):4181–4186. doi: 10.3892/mmr.2018.8375

Figure 2.

Figure 2.

Hypoxia inhibits miR-150 expression in HCMs. (A) RT-qPCR analysis of miR-150 in HCMs after hypoxia treatment. (B) Detection of miR-150 expression in HCMs cells transfected with miR-150 mimics or miR-150 inhibitors by RT-qPCR. (C) MTT was performed to measure cell viability in HCMs transfected with the miR-150 mimics or inhibitors in the present of hypoxia. (D) Flow cytometry and (E) quantification of hypoxia-induced apoptosis in HCMs transfected with the miR-150 mimics or inhibitors. Data are expressed as the mean ± standard error (n=3/group). *P<0.05, **P<0.01, ***P<0.001 vs. control group; #P<0.05, ##P<0.01 vs. hypoxia single treatment group. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; HCM, human cardiomyocyte; Con, control; PI, propidium iodide; miR, microRNA.