Figure 1.

TBMS1 (10 µM) could significantly reduce cell growth and upregulate miR-126-5p expression. (A) NCI-H1299 cells were seeded in 96-well plates and exposed to increased concentrations of TBMS1 (0, 2.5, 5, 10, 25, 50 µM) for 48 h, followed by CCK-8 assay. ***P<0.001 vs. non-TBMS1 treated control. (B) RT-qPCR analysis of miR-126-5p in NSCLC tissues and paraneoplastic tissues. compared with para-NSCLC, ***P<0.001. (C) RT-qPCR analysis of miR-126-5p in NCI-H1299 cells with 10 µM TBMS1 treatment for 48 h. Non-TBMS1 treated control was used as negative control, and 5-FU treated cells was served as positive control. β-actin was used as an internal control. Above experiments were performed for three times. Data are expressed as mean ± SD. ***P<0.001 vs. control. TBMS1, tubeimoside-1.