Table I.
Primer sequences.
| Gene | Sequence (5′->3′) | Product length, bp |
|---|---|---|
| OCN | 227 | |
| F | ACTCCATCTTCGTGCTCCTCA | |
| R | TCCGGACAGGAGAAAGGGTC | |
| ALP | 281 | |
| F | CTGTGCCTCAGCCAGCTC | |
| R | GGAGGATTCCAGAGGGGAGT | |
| RUNX2 | 225 | |
| F | CGCCTCACAAACAACCACAG | |
| R | TCACTGTGCTGAAGAGGCTG | |
| Aggrecan | 109 | |
| F | GTTTCCACAAGGGAGAGAGGG | |
| R | GTAGGTGGTGGCTAGGACGA | |
| Sox-9 | 110 | |
| F | AGGAGAACCCCAAGATGCAC | |
| R | GAGGCGTTTTGCTTCGTCAA | |
| COL2A1 | 241 | |
| F | GGTCCTGCAGGTGAACCC | |
| R | GAGGACCTCTAGGGCCAGAA | |
| adipoQ | 294 | |
| F | ATTCGGCACGAGGGATGCTA | |
| R | GCCCTTCAGCTCCTGTCATT | |
| aP2 | 181 | |
| F | TGAAAGAAGTGGGAGTGGGC | |
| R | CCTTTCCTGTCATCTGCGGT | |
| PPARγ | 123 | |
| F | GTGGAAGGCGAGCAGATGAT | |
| R | GGCAGATCTGGACTGGTAGC | |
| MMP13 | 230 | |
| F | CATGAGTTCGGCCACTCCTT | |
| R | CCTCGGAGACTGGTAATGGC | |
| COL10A1 | 143 | |
| F | CCAGCACGCAGAATCCATCTGA | |
| R | CTTGGTGTTGGGTAGTGGGC | |
| COX-2 | 297 | |
| F | TGTGAAAGGGTGTCCCTTCG | |
| R | AGTACAACACAGGAATCTTCACA |
F, forward; R, reverse.