Figure 4.

Effects of GaTx2 on OL differentiation. (A) MBP, MAG and CNP protein expression levels were assessed by western blotting. Control cells were treated with PDGF-AA for 1 day and T3 was added in for 3 days. In the experimental group, cells were treated with T3 together with GaTx2 (2 nM for 72 h). (B) Immunocytochemical staining of MBP and Olig2 indicated that GaTx2 may inhibit OL differentiation. (C) Immunocytochemical staining of Ki67 indicated that GaTx2 had no effect on OPC proliferation. The experiment was repeated four times. Scale bar, 40 µm. ***P<0.001 vs. Ctrl. OLs, oligodendrocytes; MBP, myelin basic protein; MAG, myelin-associated glycoprotein; CNP, 2′,3′-cyclic nucleotide 3′-phosphodiesterase; T3, triiodothyronine; Olig2, oligodendrocyte transcription factor 2; Ki67, marker of proliferation Ki67; OPCs, oligodendrocyte precursor cells; Ctrl, control.