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. 2018 Jan 18;17(3):4540–4546. doi: 10.3892/mmr.2018.8458

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Copyright: © Lee et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Expression of TrioBP in glioblastoma cell lines. (A) For immuno-blot analysis with anti-TrioBP and anti-actin antibodies, cell lysates were isolated from 4 established GBM cell lines (U87-MG, U251-MG, and U343-MG) and one established non-cancerous cell line (293A). The results are representative of 3-independent experiments (top panel). Relative density was obtained by densitometry of the corresponding immunoblot data. Relative and statistical differences of TrioBP expression were determined by normalizing values for actin in each lane and set the values for 293A as 1 (bottom panel). The results are presented as means ± SD. of three independent experiments. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test (*P<0.05, **P<0.01 vs. 293A). (B) Extracted total RNA from each GBM cell line was analyzed using human TrioBP specific primer sets by real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR), as described in the Materials and Methods section. The results are presented as means ± SD of three independent experiments. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test (***P<0.001 vs. 293A).

Figure 2. — Expression of TrioBP in glioblastoma cell lines. (A) For immuno-blot analysis with anti-TrioBP and anti-actin antibodies, cell lysates were isolated from 4 established GBM cell lines (U87-MG, U251-MG, and U343-MG) and one established non-cancerous cell line (293A). The results are representative of 3-independent experiments (top panel). Relative density was obtained by densitometry of the corresponding immunoblot data. Relative and statistical differences of TrioBP expression were determined by normalizing values for actin in each lane and set the values for 293A as 1 (bottom panel). The results are presented as means ± SD. of three independent experiments. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test (*P<0.05, **P<0.01 vs. 293A). (B) Extracted total RNA from each GBM cell line was analyzed using human TrioBP specific primer sets by real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR), as described in the Materials and Methods section. The results are presented as means ± SD of three independent experiments. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test (***P<0.001 vs. 293A).