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. 2015 Mar 17;23(6):7734–7754. doi: 10.1364/OE.23.007734

Fig. 1.

Fig. 1

(a) Schematic illustration of the MF PolScope. The polarization controller (LC universal compensator) was placed in the illumination path on top of the condenser lens. An analyzer was inserted in one slot of the filter cube turret, for use in birefringence imaging. MFM optics were appended to one of the side ports. (b) MFM optics form an instant 3D image consisting of a simultaneously formed focal stack of 2D planes, laid out in a tile pattern on the camera and captured in a single snapshot. (c) The multi-focus grating is placed in a secondary Fourier (objective pupil) plane formed by Relay Lens 1. The final (multi-focus) image is formed on the camera by Relay Lens 2. (d) To acquire polarization information, a series of images with the sample illuminated by light of different polarization states is recorded. Here we show the orientations used to form a birefringence image. This layout is for monochromatic imaging. For the chromatically corrected system, see Fig. 2.