Fig. 7.

Two time-points from an extended volume 25-plane multifocus movie showing a developing C. elegans embryo inside an adult worm, recorded at one frame per second. Polarization imaging is used to obtain contrast, revealing chromatin and spindles rearranging during the first cell division into the two-cell stage. This movie and an additional movie of another embryo undergoing cell division from two- to four-cell stage are included in downsized, compressed movies as Media 4 ^{(6.3MB, MOV)} and Media 5 ^{(6.3MB, MOV)} . Cropped but less compressed movies of selected focal planes containing the spindles are included in Media 6 ^{(6.8MB, MOV)} and Media 7 ^{(8.8MB, MOV)} . The size of each image tile (focal plane) is 55 μm × 55 μm laterally and the focus step between planes is 1.5 μm, providing an imaging depth of 38 μm covering the entire embryo.