Extended Data Figure 6. D3 and D14 protein levels are unaffected by GR24 treatment.

a, Phenotypes of mature transgenic plants of D3-GFP and D14-GFP in the d3 or d14 background, respectively, showing that D3–GFP and D14–GFP can rescue the corresponding phenotypes of d3 and d14. Scale bar, 10 cm. Five independent transgenic lines were shown to have similar phenotypes. b, Comparison of the tiller number of transgenic plants of D3-GFP/d3 and D14-GFP/d14. Values are means ±s.d. (n = 5). The double asterisks represent significant difference determined by the Student’s t-test at P <0.01. c, D3–GFP abundance in d3 treated with 10 μM GR24 for 60 min. Protein level was analysed by immunoblotting using GFP antibody. d, The D14–GFP protein level in the d14 background as analysed by immunoblotting using GFP antibody. Transgenic plants of D14-GFP in the d14 background were treated with 10 μM GR24 for 60 min. Rice plants (T₂ generation) were grown in the field in Hainan province.