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Determination of D53 antibody specificity by D53–GFP transgenic calli. Left, anti-GFP (Roche), 1:10,000 dilution; right, anti-D53 (1:10,000). Horseradish-peroxidase-conjugated anti-rabbit IgG and anti-mouse IgG antibodies were used as secondary antibodies to detect anti-D53 and anti-GFP.
