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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: J Neuroimmune Pharmacol. 2017 Sep 26;13(1):100–112. doi: 10.1007/s11481-017-9766-1

Fig. 2.

Fig. 2

MPP+-induces tryptase/BSSP-4 release from Wt BMMCs, and tryptase release CCL2 from astrocytes and glia/neurons. (A) Wt BMMCs were incubated with MPP+ (10 μM) and the culture media were used for tryptase assay by ELISA (n=4). MPP+ induced significant tryptase/BSSP-4 release from BMMCs as compared to untreated control cells. (B) Wt mouse neurons and Wt BMMCs co-cultured cells were treated with MPP+ (10 μM) for 24 h (n=4) and the tryptase released in the medium was assayed. MPP+ released significantly more tryptase in this co-culture system as compared to co-cultured control cells (*p<0.05; t test, control vs treated). (C, D) Wt mouse astrocytes (n=6) or glia/neurons (n=4) were incubated with tryptase/BSSP-4 (0.5–10 ng/ml) and the release of CCL2 was assayed in the culture media by ELISA. Tryptase/BSSP-4 induced CCL2 release from the astrocytes and glia/neurons. Results were shown as mean ± SEM (*p<0.05; control vs treated, C= One-way ANOVA and Tukey-Kramer post hoc, D = t test).