Fig. 7.

GMF, MPP⁺, MMCP-6, MMCP-7 and tryptase release MMP-3 from Wt brain cells or mast cells. (A, B) BMMCs grown from Wt mice were incubated with GMF (100 ng/ml) or MPP⁺ (20 μM) for 24 h and MMP-3 release in the cell culture media was measured by ELISA. (A) GMF (n=3) and (B) MPP⁺ (n=4) significantly increased the release of MMP-3 when compared with non-treated control BMMCs (*p<0.05; t test, control vs treated). (C) Wt mouse BMMCs plus glia/neurons incubated with MPP⁺ significantly induced MMP-3 release as compared to non-treated control cells (n=3, *p<0.05; t test, control vs treated). (D) Wt mouse glia/neurons were then incubated with MPP⁺ (15 μM) or GMF (100 ng/ml) for 24 h and the release of MMP-3 was measured in the culture media (n=3). MPP⁺ and GMF significantly induced the release of MMP-3 from glia/neurons. Wt glia/neurons and BMMCs were co-cultured and then stimulated with GMF. GMF augments the release of MMP-3 in this co-culture system as compared to single glia/neurons culture condition (*p<0.05; t test, control vs treated). (E, F, G) Wt mouse astrocytes or glia/neurons were incubated with MMCP-6, MMCP-7, MPP⁺ or tryptase/BSSP-4 and the release of MMCP-3 in the culture media was measured by ELISA. MMCP-7 (n=6), MMP-6 (n=6), MPP⁺ (n=6) and tryptase/BSSP-4 (n=3)-induced significantly increased MMP-3 release as compared to control cells. Results were presented as mean ± SEM (*p<0.05 control vs treated cells, One-way ANOVA and Tukey-Kramer post hoc).