Fig 6.
Effect of inhibitors on cell migration (A) and cell adhesion (B) in MDA-MB-231. Intact cells (5 x 105 cells/mL) were incubated for 1 h at 37°C in a Boyden Chamber Assay™ migration (A) or in a adhesion chamber (B) in the presence or absence (control) of inhibitors indicated in the abscissa: ouabain (1 mM), furosemide (1 mM), Monensin (100 μM) and PFA (5 mM). In the presence of these inhibitors at their respective concentrations, the cells remained viable throughout the experiment. The results are the means ± SE of at least 3 experiments, with different cell suspensions. Asterisks mark significant differences (p≤ 0.05) from control, as determined by One-Way analysis of variance (ANOVA), using Turkey’s multiple comparisons test.