Table 3.
Primers used for cloning Tmem132d promoter fragments into the luciferase vector
| Primer | Orientation | Primer sequence (5’ → 3’) |
|---|---|---|
| RVprimer3 | Forward | CTAGCAAAATAGGCTGTCCC |
| GLprimer2 | Reverse | CTTTATGTTTTTGGCGTCTTCCA |
| Tmem132d | Forward | GCAGGTACCCAAGGCTCTGCGGAGCAGTG |
| Tmem132d | Reverse | GCTAGCAATTTCTCTCTCTTCCTCTCTCCC |
| Tmem132dreverse310 A sub | Forward | GTTAGGGGTTCCTGAACTGTCCTTGCCTGAAG |
| Tmem132dreverse310 A sub | Reverse | CTTCAGGCAAGGACAGTTCAGGAACCCCTAAC |
| Tmem132dreverse310 del | Forward | GACGGTACCTCAGGGACAGGAATTTGAGG |
| Tmem132dreverse519 A sub | Forward | GTGTGAGTTCGCCTTAGATACCCTGGAAGG |
| Tmem132dreverse519 A sub | Reverse | CCTTCCAGGGTATCTAAGGCGAACTCACAC |