Fig. 2.

Upregulation of FEZ1 during OL differentiation in culture and myelin development in the optic nerve. a Representative immunoblot of FEZ1 protein in purified rat OPCs that underwent proliferation (Pro) or 4 days of differentiation in culture (Dif4). b Quantification of FEZ1 protein levels on immunoblot of primary OPC cultures (n = 4, Welch’s correction was applied). c Quantification of FEZ1 mRNA in primary OPC cultures by RT-qPCR (n = 4). d Representative immunoblot of FEZ1 protein in optic nerves on postnatal day 14 (P14) and day 30 (P30). e Quantification of FEZ1 protein levels on immunoblot of optic nerves (n = 4). f Quantification of FEZ1 mRNA in optic nerves by RT-qPCR (n = 5). β-actin was used as a loading control