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. 2017 Dec 18;7:1293. doi: 10.1038/s41398-017-0028-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — The cooperation between HDAC-dependent histone acetylation and transcription factors controls FEZ1 expression in OL. a, b FEZ1 mRNA levels in primary cultured OPCs that underwent 5 days of differentiation (DIV5; a; n = 4) and CG4 cells b (n = 5) were treated with TSA or DMSO for 24 h. c Western blot shows comparable efficiency of acH3K9 immunoprecipitation in DMSO or TSA-treated CG4 cells. d Representative illustration of increased acH3K9 at the FEZ1 promoter upon TSA treatment based on ChIP–PCR (upper panel) in rat OL and CG4 cells. ChIP–qPCR quantification of acH3K9 at the FEZ1 promoter in CG4 cells was normalized to the total input of genomic DNA and was graphically displayed (lower panel, n = 7). e, f RT-qPCR quantification of transcription repressor (FOXG1, ID2, ID4) mRNAs e and transcription activator (SOX10, TCF4, SOX6) mRNAs f in DMSO-treated or TSA-treated CG4 cells (n = 5, Welch’s correction was applied). g Representative immunoblot of Flag-ID4 expressed in CG4 cells (upper panel), which leads to downregulation of the FEZ1 mRNA detected by RT-qPCR (lower panel, n = 6 in CTL, n = 8 in Flag-ID4). h Representative immunoblot of Flag-SOX10 in CG4 cells (upper panel), which increases FEZ1 mRNA detected by RT-qPCR (lower panel, n = 5). i Expression of Flag-ID4 in CG4 cells leads to downregulation of the activator TFs (SOX10, TCF4, SOX6) mRNAs detected by RT-qPCR (n = 8 in CTL, n = 6 in Flag-ID4)