Fig. 3. Loss of Asxl1 in the BM niche alters HSC/HPC pool.

a Frequencies of LT-HSC, ST-HSC, and MPP cell populations in BM reconstituted CD45.1⁺/LSK cells of OsxCre;Asxl1^fl/fl recipients are shown (n = 3 mice per genotype). b Absolute numbers of LT-HSCs, ST-HSCs, and MPP cells from Asxl1^fl/fl and OsxCre;Asxl1^fl/fl recipients are shown (n = 3 mice per genotype). c Flow cytometric analyses of CMP, GMP, and MEP populations in the reconstituted CD45.1⁺/LKS⁻ cells from BM of representative Asxl1^fl/fl and OsxCre;Asxl1^fl/fl recipients. d Quantification of the percent CMP, GMP, and MEP populations in BM reconstituted CD45.1⁺/LKS⁻ cells of OsxCre;Asxl1^fl/fl recipients are shown (n = 3 mice per genotype). e Flow cytometric analysis of Gr1⁺/Mac1⁺ cell populations in PB of representative Asxl1^fl/fl and OsxCre;Asxl1^fl/fl recipients. f, g Quantification of Gr1⁺/Mac1⁺ cell populations in PB (f) and spleen (g) shows increased myeloid cells in OsxCre;Asxl1^fl/fl recipients (n = 4 mice per genotype). Data represent mean ± s.e.m., ns, not significant, *P < 0.05, **P < 0.01