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. 2018 Jan 23;4:4. doi: 10.1038/s41421-017-0004-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Nuclear extracts of WT BMSCs transduced with FLAG-tagged ASXL1 immunoprecipitated (IP) with anti-FLAG or non-immune IgG and probed for FLAG, POLR2A, POLR2B, and POLR2C. b Reciprocal IP and western blotting confirmed interaction of ASXL1 with POLR2A, and POLR2B. Nuclear extracts were subjected to IP using POLR2A antibody. c Gel filtration analysis of nuclear extracts from FLAG-ASXL1 overexpressing cells. ASXL1 and RNAPII complex were co-eluted from a Superose 6 column as analyzed by western blotting. The numbers over the lanes represent the eluted fraction numbers