Fig. 2. MOR association with HINT1 and NR1 C1 subunits in postmortem human prefrontal cortices of schizophrenic and depressive subjects: a comparative study vs. controls.

The MOR was immunoprecipitated and its association with HINT1 (a) and NR1 C1 subunits (b) was determined by western blotting. The regions of the blotting membrane incubated with different antibodies are indicated. The levels of anti-MOR IgG light chain (IgG lc) were used as a loading control. Representative blots are shown. Left, S/C/D triplet 9; right S/C/D triplet 10 (see Supplementary Table S1 and Fig. S1). Data expression and analyses as in Fig. 1. The MOR-associated HINT1 and NR1 C1 were compared to total HINT1 (c) and NR1 C1 (d) content, respectively. Inset: diagram showing the presence of MORs and their association with HINT1 and NMDAR NR1 C1 subunits relative to the total content of these proteins in the study groups. *, ^Ф p < 0.05 vs control in LSD post-hoc analyses