Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Feb 2;26(12):1878–1889. doi: 10.1177/0963689717739718

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution 4.0 License (http://www.creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

PMC Copyright notice

Fig. 1. — Generation of new fusion luciferase (luc2) reporters with near infrared fluorescence proteins. (A) Schematic drawing of the luc2 fusion with iRFP720 via a 14-aa linker. Photinus pyralis luc2 (PDB ID code 1LCI) and Chromophore-binding domain (CBD) of bacterial phytochrome RpBphP2 (PDB ID code 4E04) were used. (B) Western blot results using an anti-luc2 primary antibody on human embryonic kidney (HEK)-293 lysates used for transient expression of the proteins. The band in the first lane corresponds to luc2 protein (62 KDa), the second to the TurboLuc protein (∼88 KDa). The third and fourth lanes correspond to luc2-iRFP670 (abbreviated pLuc670) and luc2-iRFP720 (abbreviated pLuc2720; ∼97 KDa). (C) Immunofluorescence for the analysis of colocalization of luc2 (green signal) and near infrared proteins (red signal) in HEK-293 cells. Nuclei are stained with 4′,6-diamidino-2-phenylindole (blue signal). luc2 was detected using an anti-luc2 antibody and Fluorescein isothiocyanate (FITC)-labeled secondary antibody. Near infrared proteins were detected using a 633-nM excitation laser. Image on the left corresponds to cells expressing luc2-iRFP670 and image on the right corresponds to cells expressing luc2-iRFP720.