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. 2018 Feb 2;26(12):1878–1889. doi: 10.1177/0963689717739718

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© The Author(s) 2018

This article is distributed under the terms of the Creative Commons Attribution 4.0 License (http://www.creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 5. — Serial dilutions of luc2-iRFP720 expressing human mesenchymal stem cells (hMSCs) measured for bioluminescence and fluorescence intensity. (A) Bioluminescence imaging (BLI) was measured for the serial dilution of of luc2-iRFP720 expressing hMSCs (0, 3.125, 6.250, 12.500, 25.000, and 50.000 cells; r ² = 0.9921). Data are represented as mean (SD) (n = 3). (B) fluorescence imaging (FLI) was measured for the serial dilution (0, 3.125, 6.250, 12.500, 25.000, 50.000, and 100.000 cells) at 700 nm (r ² = 0.9980) using the Odyssey scanner. Data are represented as mean (SD) (n = 6). (C) BLI of decreased amount of cells (1 × 10³, 1 × 10⁴, 1 × 10⁵, and 1 × 10⁶). Images were taken 20 min after injection of D-luciferin (25 µM/kg). (D) FLI of decreased amounts of cells (1 × 10³, 1 × 10⁴, 1 × 10⁵, and 1 × 10⁶) when injected into the mouse brain using Pearl Imager with 700 nm settings.