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. 2018 Feb 6;8:2494. doi: 10.1038/s41598-018-20861-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of separate cell populations with or without EGFP expression. (a) Vero cells, (b) Vero-d2EGFP cells, (c) a 1:1 ratio of Vero:Vero-d2EGFP cells, and (d) a 4:1 ratio of Vero:Vero-d2EGFP cells were subjected to cytofluorometry. The range of background fluorescence generated by parental Vero cells and a minor population of the Vero-d2EGFP cells is in black. The distribution of higher levels of fluorescence for the Vero-d2EGFP cells is in dark green, while light green highlights the lower level of fluorescence from a subpopulation of Vero-d2EGFP cells. The peak fluorescent intensity from the population of Vero-d2EGFP cells with the highest fluorescence levels in panel b is represented by the red line in all panels.