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. 2018 Feb 6;8:2494. doi: 10.1038/s41598-018-20861-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Population-wide loss of fluorescence from cycloheximide-treated Vero-d2EGFP cells. (a) Untreated parental Vero cells (black), untreated Vero-d2EGFP cells (green), or Vero-d2EGFP cells treated with cycloheximide for 4 h (blue) or 8 h (red) were subjected to cytofluorometry. (b) Using data collected from the same cells by either cytofluorometry (grey bars) or with a plate reader (black bars), signals from the cycloheximide-treated cells were expressed as percentages of the value recorded for untreated Vero-d2EGFP cells.