Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Feb 6;8:2494. doi: 10.1038/s41598-018-20861-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Dose-dependent recovery from intoxication. Vero-d2EGFP cells were incubated with various concentrations of (a) ricin, (b) Stx1, (c) EtxA, or (d) Dtx for 20 h and then chased for 48 h in the absence of toxin. Left columns: Orange, blue, and red lines were derived from cytofluorometry analysis of toxin-treated Vero-d2EGFP cells at the end of the 20 h toxin incubation or the end of the 48 h chase. Unintoxicated parental Vero cells (black lines) and unintoxicated Vero-d2EGFP cells (green lines) were also processed for each condition. Right columns: At the end of the 48 h chase, representative images of cells initially exposed to the stated toxin concentrations were taken at 200× magnification. Cell viability, as assessed by MTS assay (n = 3, avg. ± std. dev.) or annexin V (AV) / 7-AAD staining of the same cell population processed for EGFP cytofluorometry, is indicated. These experiments were performed at the same time as the data presented in Fig. 5.