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. 2018 Feb 6;8:2528. doi: 10.1038/s41598-018-20342-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect on the expression of dopamine receptors and its targets associated with pre and postsynaptic signaling in differentiated PC12 cells following the exposures of cadmium (10 μM) and quercetin (100 μM). Quantitative analysis revealed that PC12 cells were exposed to Cd (10 µM) and quercetin (100 µM) for 24 hr alters the pre and postsynaptic signaling targets. β-actin was used as an internal control to normalize the data. Relative protein levels were quantified using Alpha Ease FC Stand Alone V.4.0 software after normalization with β-actin. The cropped gels are displayed for the clear representation.The data represent means ± SE of three independent experiments; *p < 0.05; **p < 0.01; a-compared to control group; b-compared to cadmium exposed group; CONT – Control, Cd – Cadmium, QUR – Quercetin.