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. 2018 Feb 6;9:511. doi: 10.1038/s41467-017-02204-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commonslicense, unless indicated otherwise in a credit line to the material. If material is not included in the article’sCreative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — EF-Tu confers protection on L-Thr-tRNA^Thr(G4•U69) against ATD. Deacylation of l-Ala-tRNA^Thr(G4•U69) by different concentrations of MmATD in the presence of a unactivated EF-Tu, and b activated EF-Tu. Deacylation of l-Thr-tRNA^Thr(G4•U69) by different concentrations of MmATD in the presence of c unactivated EF-Tu, and d activated EF-Tu. Aminoacyl-tRNAs and EF-Tu were used at 200 nM and 2 µM final concentration, respectively. Error bars denote one standard deviation from the mean of three independent readings