Figure 5.
Effect of the oxidizing agent menadione and the fatty acid UDA on T. rubrum. (a) Susceptibility of T. rubrum to menadione (5 µM, 10 µM, 20 µM, 40 µM) and UDA (12.5 µg/mL and 25 µg/mL). Plates were inoculated, from left to right in each panel, with 106, 105, 104, 103, and 102 cells/mL of conidial suspension and incubated at 28 °C for 96 h. (b) qRT-PCR for T. rubrum genes encoding the oxidizing enzymes glutathione transferase, and cytochrome c peroxidase. Relative expression was assessed using 0 h as the reference sample after normalization with the rpb2 endogenous gene. Significantly different values are indicated by asterisks, as determined using ANOVA followed by Tukey’s post hoc test (P < 0.05).