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. 2018 Feb 6;8:2489. doi: 10.1038/s41598-018-20650-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Liposome leakage. Penetration of flu-OCBs into liposomes (a,b and c): (a) Fluorescence images of liposomes after being incubated with flu (free dye molecules, in green) for 0, 30 and 60 min (left) accompanied with plots of fluorescence intensity (F.I.) along the dotted line of the corresponding liposomes (right), (b) Fluorescence images of liposomes after being incubated with flu-OCBs (in green) for 0, 30 and 60 min (left) accompanied with plots of F.I. along the dotted line of the corresponding liposomes (right), (c) Plots of F.I. at the inside and outside of the liposomes, as a function of incubation time (shown as mean ± SD). Anthocyanin leak from liposomes (d,e and f): (d) Fluorescence images of anthocyanin (pseudo-color, yellow) filled liposomes after being incubated with water for 0, 30 and 60 min (left) accompanied with plots of F.I. along the dotted line of the corresponding liposomes (right), (e) Fluorescence images of anthocyanin (pseudo-color, yellow) filled liposomes after being incubated with OCBs for 0, 30 and 60 min (left) accompanied with plots of F.I. along the dotted line of the corresponding liposomes (right), (f) Plots of anthocyanin F.I. at the inside and outside of the liposomes after water addition (without OCB) or OCBs addition (with OCBs), as a function of incubation time (shown as mean ± SD).