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. 2018 Feb 6;8:2496. doi: 10.1038/s41598-018-20187-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Median-joining network and geographic distribution of Euschistus heros haplotypes in South America. (a) Network of 159 concatenated mitochondrial COI-Cytb sequences. Size of haplotype circles reflects sample size, and gray nodes represent missing haplotypes. Colors indicate the two mitochondrial lineages of haplotypes: lineage N (northern) in green, and lineage S (southern) in blue. Number of mutation steps is shown as hatch marks. (b) Geographic distributions of the mitochondrial haplotypes. Circles represent the proportion of each lineage. The map was obtained from Google Maps, Map ©2017 Google, INEGI. Pie charts were drawn with Microsoft Excel. Maps were modified with GIMP 2.8.22 (c) Network of 124 nuclear ITS1 region sequences. The haplotype color refers to the mitochondrial lineages of the individual from which the ITS1 sequence was obtained.