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. 2018 Feb 6;8:2480. doi: 10.1038/s41598-018-20842-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Examples of the time courses for the formation of LM1-LM12 obtained using trains of ten pulses of [L]_free with different peak amplitudes released at 1 Hz (A), 10 Hz (B), or 100 Hz (C). Each pulse of [L]_free had 50 ms of duration. The time courses of [L]_free are showed in Suppl. Fig. S1A–C. The control K_Ds for the interactions of M1-M12 with L are showed on the right of the panels and the k_fs for the association reactions are indicated in A. The legend indicates the colour code used to represent each concentration of [L]_free.