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. 2018 Feb 6;8:2474. doi: 10.1038/s41598-018-20841-z

Figure 2.

Figure 2

Heme binding to the GB1CBS(1–40) fusion protein determined by UV/Vis spectroscopy. (a) The GB1CBS(1–40) fusion protein was incubated with hemin at the concentrations indicated (5 μM protein, 0.32–25.45 μM hemin). (b) Titration curve observed at 421 nm.