Table 1.

de novo transcriptome assembly statistics for the SCN early life stage assembly.

Number of transcripts	147,910
Total assembly length (Mb)	46.7
Number of trinity ‘genes'	71,093
N50 (bp)	1,085
Maximum contig size (bp)	11,112 bp
Minimum contig size (bp)	201 bp
Average contig length (bp)	658 bp
Predicted proteins	78,625
BUSCO score	C: 68%, F: 14%, M: 18%

The assembly was generated from H. glycines pre-parasitic second-stage juvenile samples as well as parasitic second-stage juvenile samples from susceptible and resistant host interactions using the Trinity de novo transcriptome assembly tool. The transcriptome was assessed for completeness using the tool BUSCO (benchmarking universal single-copy orthologs) to identify complete (C), fragmented (F), and missing (M) sequences representing conserved orthologs found in all eukaryotes.