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. 2018 Feb 6;8:2477. doi: 10.1038/s41598-018-20821-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of KD025 on the expression of adipogenic and lipogenic genes. 3T3-L1 cells were differentiated through incubation in DMI with or without KD025 (5 µM) for the indicated time points and mRNA expression was measured by real time PCR. (A) Lipogenic genes: Fabp4, Slc2A4, and Srebp1. *p < 0.05; **p < 0.01; ***p < 0.001 vs. the corresponding control condition. (B) Pref1 and early activated genes: Cebpb and Cebpd. The data are the representative from more than three independent experiments. Data are expressed as means ± S.E. based on triplicate.