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. 2018 Feb 6;8:2477. doi: 10.1038/s41598-018-20821-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of KD025 on actin cytoskeleton during adipogenesis. (A) 3T3-L1 cells were differentiated through incubation with DMI in the presence of 10 µM of KD025, Y-27632, or fasudil. Cells were fixed on day 1, 3, or 8 after the start of differentiation, and were probed for F-actin (green) using phalloidin and the nucleus (blue) with DAPI. Pre-adipocyte was stained for comparison (left). The horizontal bar represents 50 μm. (B) The level of total F-actin was measured and represented in arbitrary units. The average intensity level per cell was derived by obtaining the sum of F-actin intensity from multiple independent images and then by dividing the sum with total cell number (n ≥ 80). Data are expressed as means ± S.E.