Skip to main content
. 2018 Feb 6;8:2487. doi: 10.1038/s41598-018-20531-w

Figure 5.

Figure 5

(A) Effect of QC treatment on exogenous pcDNA-p21-Flag expression. Transiently transfected NTC and p62 shRNA OV2008 cells with empty vector and pcDNA-p21-Flag vector. Cells were exposed to QC (5 and 10 µM) for 24 hours. Cells were harvested and subjected to Western blot analysis using anti-Flag and beta-actin antibodies. (B) C13 NTC and ATG5 knockdown (HBF2) C13 cells were treated with Cyclohexamide (20 µg/ml) for 0, 0.5, 1, 2, 4 and 6 hours. Cell lysates were subjected Western blot analysis using anti-p62/SQSTM1, Skp2, p21 and anti-GAPDH antibodies. (C) C13 NTC and p62 knockdown cells were treated with Cyclohexamide (20 µg/ml) for 0, 0.5, 1, 2, 4 and 6 hours. Cell lysates were subjected Western blot analysis using anti-p62/SQSTM1, Skp2, p21, p27 and anti-GAPDH antibodies.