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. 2018 Feb 6;8:2487. doi: 10.1038/s41598-018-20531-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Western blot analysis of Skp2, p21 protein expression in NTC and Skp2 shRNA clones in C13, OV90 and Skov3ip1. (B) OV90 NTC and Skp2 shRNA1 (HAX49), shRNA2 (HAX50) were exposed with QC at 5 and 10 µM for 24 hours. Cells were harvested and subjected to Western blot analysis using anti-p27, anti-p21, anti-LC3B, anti-cleaved PARP, and anti-GAPDH antibodies. (C) Skovip3 NTC and Skp2 shRNA1 (HAX49), shRNA2 (HAX 50) were exposed with QC at 5 and 10 µM for 24 hours. Cells were harvested and subjected to Western blot analysis using anti-p62/SQSTM1, anti-Skp2, anti-p21, anti-p27, anti-LC3B, anti-cleaved PARP, anti-PDI and anti-GAPDH antibodies.