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. Author manuscript; available in PMC: 2019 Jan 24.
Published in final edited form as: J Am Chem Soc. 2018 Jan 11;140(3):900–903. doi: 10.1021/jacs.7b12369

Figure 2.

Figure 2

A) Equilibrium fluorescence spectra of ht-ADH W87F (2 μM) with ligands NADH (20 μM) and isobutyramide (100 mM) in potassium phosphate buffer (50 mM, pH 7) excited at 280 nm. FRET between W49 and NADH is strongest in the ternary complex. B) Temperature dependent fluorescence spectra of ht-ADH W87F:NADH:isobutyramide. The inset shows the integrated NADH emission (420 – 460 nm) vs temperature.