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. 2018 Feb 7;13(2):e0192214. doi: 10.1371/journal.pone.0192214

Fig 2. Inhibition of Igf signaling does not affect autophagy activation in the regenerating muscle.

Fig 2

GFP-LC3 (A and D) fish were myectomyzed and LysoTracker Red (B and E) was used to label autophagy in the regenerating LR in fish treated with DMSO (A-C) or BMS754807 (D-F). C and F show the merging of A-B and D-E, respectively. GFP-LC3 (G) and LysoTracker (H) fluorescence intensity of the regenerating muscle were measured and no statistically significant difference between DMSO and BMS754807 treated fish was found. Values represent average ± SD (Student’s t-test, significance set at P < 0.05, n = 5). P, pituitary.