Fig 2. Effects of L-Arg, L-Cit and LALC on ROS generation in HUVECs.

Cells were incubated with NG or HG for 3 days with or without L-Arg (300 μM) or L-Cit (300 μM). (A) ROS generation was detected as intracellular oxidant generation by flow cytometry (n = 6). (B) Typical western blots for p22^phox, p47^phox and p67^phox, a subunit of NADPH oxidase, are shown. (C-E) A summary of the quantification of densitometric measurements of the immunoblot data. The data were normalized to ß-actin (n = 3–5). ##P<0.01, #P<0.05 versus NG. **P<0.01, *P<0.05 versus HG. Data are given as the mean ± SD from at least three independent experiments.