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. 2018 Feb 7;13(2):e0192559. doi: 10.1371/journal.pone.0192559

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© 2018 Benforte et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — A. Aggregation assay at 30°C of the wild type (wt), wapH and complemented strain (pSEVAwapH). Values represent mean ± SD of 5 independent measurements. B. Aggregation assay with different strains expressing mCherry protein and mixed with an unmarked strain. Values represent media ± SD of 5 independent measurements. C. Microscopic visualization of mixed aggregates using cells grown at 30°C or from cold shock experiments. Strains expressing fluorescent proteins were mixed and settled for15 min. An aliquot from the bottom of the tube was taken and aggregates were observed in a confocal microscope using 1000X magnification. Representative images from triplicate independent experiments are shown.