Fig 4. Nanomechanical determinations using atomic force microscopy (AFM) in live and hydrated cells.
A Surface to Volume ratio (S/V) was determined using Gwyddion software. B. Cell elasticity determination at different culture conditions. Force-distance curves were obtained using MultiMode 8 with a Nanoscope V controller, Bruker in contact mode for at least 10 cells per condition in 10 different points along the major axis per triplicate. Adjustment to the Sneedon model was performed between 0 and 2 nN and the Young module was calculated. C. Kbacterium determinations as a measure of bacterial turgor pressure. Adjustment to the Hooke’s law was performed between 2 and 4 nN in the same curves Force-distance described above. Wild type (wt), wapH and complemented strain (pSEVAwapH).