Figure 6.
NK cell subsets quantified using either bismuth- or lanthanide-tagged CD56 antibodies. (a) Comparable frequencies of NK-cell subsets determined using either 209Bi- or 176Yb-tagged CD56 antibodies in human PBMCs from three healthy donors. NK cells are gated as Supporting Information S4, and three NK-cell subsets are gated as CD56brightCD16−, CD56dimCD16+/−, and CD56−CD16+. (b–d) Comparable frequencies of NK-cell subsets in each donor, depicted with biaxial scatter plots. (Pearson correlation, r = 0.999, P < 0.00001; Supporting Information Figure S5).