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. 2018 Feb 7;6(3):e13579. doi: 10.14814/phy2.13579

Figure 2.

Figure 2

Acute WPS exposure induces pulmonary inflammation characterized by increased neutrophils and T‐lymphocytes in the BAL fluid. The inflammatory influx of (A) total cell counts, (B) macrophages, (C, E, and G) neutrophils, and (D, F, and H) lymphocytes into the BAL fluid of different strains of mice in response to 10 days WPS exposure. Total cells counts/mL were determined in BAL fluid by AO/PI staining using a cellometer. A total of 500 cells were counted to determine the total number of macrophages, neutrophils and lymphocytes on slides stained with Diff‐Quik. Data are represented as means ± SEM (n = 8 per group) and significance determined using two‐way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001 versus air.