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. 2018 Feb 7;8:2551. doi: 10.1038/s41598-018-20793-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ion intensity maps of select analytes associated with SVZ layers. Spatial distributions are shown for select lipid ions that were identified as statistically co-localised with the ependymal layer (a), the hypocellular layer (b) and the myelin layer (c), respectively, when compared to the three other SVZ layers in each circumstance. Lipid signal intensities in each MALDI sampling position are scaled to the maximum signal intensity for each lipid across all sampling positions, with mild denoising applied to the images within the SCiLS Lab software. The positions of the ependyma of the lateral wall (LW) and the medial wall (MW) of the ventricle are indicated within post-MALDI HE-LFB stains. Scale bar: 250 µm. PC = phosphatidylcholine, CL = cardiolipin, SM = sphingomyelin, PI-Cer = ceramide phosphoinositol.