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. 2018 Feb 7;8:2577. doi: 10.1038/s41598-018-20936-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Micro-RNA enrichment in captured EVs determined by RNAseq. (a) Heat map of hierarchical clustering of 109 miRNAs (All species) differentially enriched in CD47⁺ versus CD47⁻ EVs at p < 0.05. (b) 27 miRNAs were upregulated, and 5 hsa-miRNA are enriched only in CD47⁺ EVs. (c) A comparison of CD47⁺ and CD63⁺ EVs shows differential expression of 100 miRNAs (All species) at p < 0.05 using log base 2 of total RPM, (CD47_CAP and CD63_CAP with Student’s t-test), and 11 hsa-miRNAs are presented in the graph. (d) A comparison of CD47⁺ and MHC1⁺ EVs shows differential expression of 112 miRNAs (All species) at p < 0.05 using log base 2 of total RPM (CD47_CAP and YCD63_CAP with Student’s-test), and 21 hsa- miRNA are presented in the graph.