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. 2018 Feb 7;8:2534. doi: 10.1038/s41598-017-18062-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotypic analysis of CMV-specific CD8⁺ T-cells in healthy donors. (A) Percent frequencies of NLV/HLA-A*0201 tetramer⁺ (black dots) and NLV/HLA-A*0201 tetramer⁻ CD8⁺ T-cells (white dots) displaying the indicated differentiation phenotypes in healthy donors (n = 6). (B) Percent frequencies of NLV/HLA-A*0201 tetramer⁺ (black dots) and NLV/HLA-A*0201 tetramer⁻ CD8⁺ T-cells (white dots) expressing the indicated markers in healthy donors (n = 6). Statistical comparisons were performed using the Mann-Whitney U test. **P < 0.001, *P < 0.01. Horizontal bars indicate mean values. N, naïve; CM, central-memory; EM, effector-memory; E, effector.