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. 2018 Feb 7;8:2564. doi: 10.1038/s41598-018-20981-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative expression levels of seven P450 unigenes in imidacloprid-treated B. odoriphaga. (a) Four upregulated P450s unigenes; (b) Three downregulated P450 unigenes. The transcription levels of the seven P450s unigenes were determined by quantitative real time PCR. Each bar indicates the mean of three biological replicates (±SE). 18 s was used as a reference gene. The different letters on the bars represent significant differences between treatment and control group based on Duncan’s multiple comparison test (P < 0.05). Numbers in each bar represent fold change compared with the control.