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. 2018 Feb 7;8:2564. doi: 10.1038/s41598-018-20981-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The dsRNA-mediated suppression of P450s transcript expression in B. odoriphaga fed on the artificial diet with dsRNA. The control group was fed with dsGFP. (a) Repressions of the transcripts of four P450s genes after larvae were fed an artificial diet with dsRNA for 48 h. The transcript levels of four P450s genes were examined using qRT-PCR, and 18 s was selected as a reference gene. (b) Insecticide bioassays were conducted 48 h after the uptake of P450 dsRNA by the standard contact and stomach bioassay method. Mortality was assessed 48 h after the insecticide treatments. Results are mean ± SE of three biological replications (n = 3). The asterisks on the bars indicate significant differences between the control and treatment group after P450s dsRNA uptake (Student’s t-test, P < 0.05).