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. 2018 Feb 7;8:2586. doi: 10.1038/s41598-018-21049-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histologically on DNFB-induced dermatitis, contact dermatitis was induced in NC.h2^b/nc and NC.h2^b/b while AD-like lesions were induced in NC/Nga. (A) Thickening of the epidermis (black arrow) and the perivasculitis of the dermis (blue arrow) in upper panel (skin) are indicated. Thickening of the epidermis (blue arrow) and dermis (black arrow) in lower panel (ear) are indicated. Partially extended thickening of the dermis is highlighted (red arrow). Scale bar corresponds to 100 µm in all images of (A). Against the infiltration of eosinophils in the dermis (B) and in perivascular (C), and the TSLP expression in epidermis (D) in NC/Nga, the infiltration of lymphocytes and plasma cells in the dermis (E) and in perivascular (F), and the TSLP expression in perivascular (G) in NC.h2^b/b is indicated. Scale bar in (B), (C), (E), (F) represents 25 µm. Scale bar in (D) and (G) is 50 µm.